Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary Targetanthrax LF metalloprotease

Target K_i: 1 nM against Anthrax lethal factor (LF) metalloprotease

Cell permeable: yes

General description

A cell-permeable N-acetylated, C-hydroxamate derivative of a 14-mer peptide designed from the MEK-2 template that acts as a competitive inhibitor of Anthrax lethal factor (LF) metalloprotease (K_i = 1 nM). Also inhibits MEK-3 cleavage. Protects against Anthrax toxin induced cytotoxicity in RAW264.7 and J772.A1 cells.

A cell-permeable derivative of a 14-mer peptide that is based on the MEK-2 template and acts as a competitive inhibitor of Anthrax lethal factor metalloprotease (K_i = 1 nM). Also inhibits MEK-3 cleavage. Exhibits protective effects against Anthrax toxin-induced cytotoxicity in RAW264.7 and J772.A1 cells. Reported to competitively inhibit furin (K_i = 49 nM.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Peinado, J.R., et al. 2004. Biochem. Biophys. Res. Commun.321, 601.Tonello, F., et. al. 2002. Nature418, 386.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Ac-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-NHOH

Warning

Toxicity: Standard Handling (A)